1PE0

Crystal structure of the K130R mutant of human DJ-1

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (1.7 Å)

Cell axes77.70678.17363.819
Cell angles90.00108.8890.00
SpacegroupC 1 2 1
Resolution limits19.54 - 1.70
the highest resolution shell value1.760 - 1.700
R-factor0.191
R-work0.19100
the highest resolution shell value0.211
R-free0.23100
the highest resolution shell value0.249
RMSD bond length0.005
RMSD bond angle1.300 (22.800*)

Data Collection Statistics

Resolution limits20.00 - 1.70
the highest resolution shell value -
Number of reflections39841
Number of measurements112333*
Rmerge_l_obs0.065
the highest resolution shell value0.264
Completeness97.8
I/sigma(I)0

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1VAPOR DIFFUSION, HANGING DROP (Vapor diffusion*)7 (8.0*)277 (4*)*

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirTris100 (mM)pH8.0
21reservoirPEG335010-12 (%(w/v))
31dropprotein15 (mg/ml)
41dropTris30 (mM)pH8.0
Annotated Information is extracted from Literature Info*