1P9E

Crystal Structure Analysis of Methyl Parathion Hydrolase from Pseudomonas sp WBC-3

Experimental procedure

実験手法	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	2003-03-29
Detector	RIGAKU RAXIS V
Wavelength(s)	1.2828, 1.2832, 0.9000
Spacegroup name	P 43 21 2
格子定数 [Å]	85.020, 85.020, 199.773
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	50.000 - 2.400
Rwork	0.218
R-free	0.25900
Structure solution method	MAD
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.673
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.380
分解能 [Å] (高)	2.300	2.300
Rmerge_l_obs	0.080	0.380
独立反射数	180629
完全性 [%]	95.9	96
冗長性	5.6	4.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	PEG400, Na-Acetate, CdCl2, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K