1OQN
Crystal structure of the phosphotyrosine binding domain (PTB) of mouse Disabled 1 (Dab1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00465 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.106, 66.481, 90.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.246 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m7e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.260 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.077 * |
| Total number of observations | 400319 * |
| Number of reflections | 16118 |
| <I/σ(I)> | 27.3 |
| Completeness [%] | 99.5 |
| Redundancy | 24.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 18 * | PEG 3350, magnesium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | protein | 20 (mg/ml) | |
| 5 | 1 | reservoir | PEG3350 | 20 (%(w/v)) | |
| 6 | 1 | reservoir | tri-lithium citrate | 0.2 (M) |
