1OI9
Structure of human Thr160-phospho CDK2/cyclin A complexed with a 6-cyclohexylmethyloxy-2-anilino-purine inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.122, 134.732, 148.244 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.100 |
| Rwork | 0.233 |
| R-free | 0.27640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h1s |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP (7.3.01) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.040 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.079 | 0.420 |
| Number of reflections | 86154 | |
| <I/σ(I)> | 6.9549 | 1.7 |
| Completeness [%] | 98.7 | 99.5 |
| Redundancy | 3.76 | 3.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.7-0.85 M POTASSIUM CHLORIDE, 1.1-1.25 M AMMONIUM SULFATE, 40 MM HEPES, PH 7.0, 5 MM DITHIOTHREITOL, 10 MG/ML PROTEIN, 8M SODIUM FORMATE CRYO-PROTECTANT |
