1OI9

Structure of human Thr160-phospho CDK2/cyclin A complexed with a 6-cyclohexylmethyloxy-2-anilino-purine inhibitor

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-14
Detector	ADSC CCD
Spacegroup name	P 21 21 21
格子定数 [Å]	74.122, 134.732, 148.244
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	100.000 - 2.100
Rwork	0.233
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h1s
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP (7.3.01)
Refinement software	REFMAC

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	36.040	2.210
分解能 [Å] (高)	2.100	2.100
Rmerge_l_obs	0.079	0.420
独立反射数	86154
<I/σ(I)>	6.9549	1.7
完全性 [%]	98.7	99.5
冗長性	3.76	3.78

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		7		0.7-0.85 M POTASSIUM CHLORIDE, 1.1-1.25 M AMMONIUM SULFATE, 40 MM HEPES, PH 7.0, 5 MM DITHIOTHREITOL, 10 MG/ML PROTEIN, 8M SODIUM FORMATE CRYO-PROTECTANT