Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 579.700, 626.650, 787.200 |
| 格子定数 [度] | 90.00, 89.90, 90.00 |
精密化法
| 残基 | 190.130 - 3.450 |
| R因子 | 0.373 |
| Rwork | 0.373 |
| R-free | 0.36300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CRYO EM RECONSTRUCTION |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 24.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AVE |
| Refinement software | CNS (1.1) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 190.000 |
| 分解能 [Å] (高) | 3.440 |
| Rmerge_l_obs | 0.160 |
| 独立反射数 | 4797606 |
| <I/σ(I)> | 9.6 |
| 完全性 [%] | 63.0 |
| 冗長性 | 1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | Wikoff, W.R., (1998) Virology, 243, 113. * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | potassium phosphate | 50 (mM) | pH7.0 |
| 2 | 1 | reservoir | PEG8000 | 1.0-1.5 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.9 (M) | |
| 4 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | 2-mercaptoethanol | 1 (mM) | |
| 7 | 1 | drop | protein | 27-30 (mg/ml) |
