1OFN

Purification, crystallisation and preliminary structural studies of dTDP-4-keto-6-deoxy-glucose-5-epimerase (EvaD) from Amycolatopsis orientalis; the fourth enzyme in the dTDP-L-epivancosamine biosynthetic pathway.

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-15
Detector	ADSC CCD
Spacegroup name	P 21 21 2
格子定数 [Å]	98.575, 71.996, 57.100
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	57.740 - 1.500
R因子	0.146
Rwork	0.144
R-free	0.17800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dzr
結合長の平均二乗偏差(RMSD) [Å]	0.018
結合角の平均二乗偏差(RMSD) [度]	1.710
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	57.735	1.550
分解能 [Å] (高)	1.500	1.500
Rmerge_l_obs	0.056	0.263
独立反射数	65259
<I/σ(I)>	7.3	2.6
完全性 [%]	100.0	100
冗長性	4.5	4.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	8		25%PEG4K, 100MM MGCL2, 100MM TRIS-HCL, PH8.0, SITTING DROP,, pH 8.00