1OAN
Crystal structure of the dengue 2 virus envelope protein
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| 格子定数 [Å] | 81.541, 81.541, 288.623 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 50.000 * - 2.750 |
| R因子 | 0.261 |
| Rwork | 0.261 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1OAM |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.415 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.850 |
| 分解能 [Å] (高) | 2.750 | 2.750 |
| Rmerge_l_obs | 0.079 | 0.409 |
| 独立反射数 | 27029 | |
| <I/σ(I)> | 21.7 | 2.5 |
| 完全性 [%] | 90.0 * | 49 * |
| 冗長性 | 10 | 1.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 8 * | 4 * | 11% PEG 8000, 1 M NACL, 0.1 M TRIS/HCL PH 9.0, 20% GLYCEROL |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | PEG8000 | 11 (%) | |
| 2 | 1 | reservoir | sodium formate | 1 (M) | |
| 3 | 1 | reservoir | glycerol | 20 (%) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) | pH8. |
| 5 | 1 | reservoir | beta-octylglucoside | 0.5 (%) |
