1OA1
REDUCED HYBRID CLUSTER PROTEIN (HCP) FROM DESULFOVIBRIO VULGARIS HILDENBOROUGH STRUCTURE AT 1.55A RESOLUTION USING SYNCHROTRON RADIATION.
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 64.053, 67.464, 134.955 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 19.500 - 1.550 |
| R因子 | 0.132 |
| Rwork | 0.131 |
| R-free | 0.15300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB FROM ANAEROBILLY AS-ISOLATED HCP PROTEIN 1.35A (D.ARAGAO PRIVATE COMMUNICATION) |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 * |
| 結合角の平均二乗偏差(RMSD) [度] | 1.292 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 19.500 | 1.590 |
| 分解能 [Å] (高) | 1.550 | 1.550 |
| Rmerge_l_obs | 0.071 | 0.254 |
| Total number of observations | 262651 * | |
| 独立反射数 | 81856 | |
| <I/σ(I)> | 13.2 | 3.6 |
| 完全性 [%] | 95.7 | 93.5 |
| 冗長性 | 3.2 | 3.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, sitting drop * | 6.5 | 277 | 25 PEG 4000 0.1M MES PH 6.5, TEMPERATURE 277 KELVIN |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | PEG4000 | 25 (%) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
| 3 | 1 | drop | protein | 25 (mg/ml) | |
| 4 | 1 | drop | Tris-HCl | 20 (mM) | pH7.6 |
| 5 | 1 | drop | 0.05 (M) |
