1O65
Crystal structure of an hypothetical protein
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795 , 0.9795 |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 72.116, 97.878, 98.670 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 50.000 - 2.330 |
| Rwork | 0.231 |
| R-free | 0.30800 |
| Structure solution method | Se-Met MAD phasing |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.100 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA) |
| Refinement software | REFMAC (4.0) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.460 |
| 分解能 [Å] (高) | 2.330 * | 2.330 |
| Rmerge_l_obs | 0.057 | 0.123 |
| 独立反射数 | 30106 | |
| <I/σ(I)> | 23.3 | 13.7 |
| 完全性 [%] | 98.9 | 98.9 |
| 冗長性 | 7.2 | 4.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 277 |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | protein | 16mg/ml | |
| 2 | 1 | 2 | NaCl | 150mM | |
| 3 | 1 | 2 | Hepes | 10mM | |
| 4 | 1 | 2 | beta-mercaptoethanol | 1mM | |
| 5 | 1 | 2 | methionine | 10mM |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | methionine | 10 (mM) | |
| 4 | 1 | drop | glycerol | 10 (%) | |
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | drop | protein | 10 (mg/ml) |
