1O16
RECOMBINANT SPERM WHALE MYOGLOBIN H64D/V68S/D122N MUTANT (MET)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-11-07 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 6 |
| 格子定数 [Å] | 91.200, 91.200, 45.850 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 18.180 - 1.950 |
| R因子 | 0.16 |
| Rwork | 0.160 |
| R-free | 0.19000 |
| Structure solution method | ISOMORPHIC WITH WILD-TYPE |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 17.000 * |
| Data reduction software | XDS |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 19.000 | 2.080 |
| 分解能 [Å] (高) | 1.950 | 1.950 |
| Rmerge_l_obs | 0.063 | |
| 独立反射数 | 14391 | |
| <I/σ(I)> | 12 | |
| 完全性 [%] | 89.7 * | 80.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 9 | pH 9.00 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | ammonium sulfate | 3.2 (M) | |
| 2 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | EDTA | 1 (mM) | pH9.0 |
| 4 | 1 | reservoir | ammonium sulfate | 2.4-2.6 (M) |
