1NZG
Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-06-08 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 25.260, 44.110, 45.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 * - 1.600 |
Rwork | 0.192 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 * | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.063 | 0.182 |
Number of reflections | 7031 | |
<I/σ(I)> | 22.9 | 6.5 |
Completeness [%] | 97.6 | 82.9 |
Redundancy | 8.2 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | MPD, Sodium Cacodylate, Spermine, Sodium Chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | Sodium Cacodylate | ||
3 | 1 | 1 | Spermine | ||
4 | 1 | 1 | Sodium Chloride | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | Sodium Chloride |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | oligonucleotide | 2000 (nm) | |
2 | 1 | drop | sodium cacodylate | 40 (mM) | pH6.0 |
3 | 1 | drop | 80 (mM) | ||
4 | 1 | drop | spermine tetrahydrochloride | 12 (mM) | |
5 | 1 | drop | 10 (%(v/v)) | ||
6 | 1 | reservoir | MPD | 35 (%(v/v)) |