1NNW
hypothetical protein from Pyrococcus furiosus Pfu-1218608
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.06 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 50.140, 50.842, 64.403 |
| 格子定数 [度] | 83.70, 81.64, 81.77 |
精密化法
| 残基 | 20.000 - 1.900 |
| R因子 | 0.21692 |
| Rwork | 0.216 |
| R-free | 0.23498 |
| Structure solution method | MIRAS |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.136 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.960 |
| 分解能 [Å] (高) | 1.890 | 1.890 |
| 独立反射数 | 46373 | |
| <I/σ(I)> | 21 | 3.6 |
| 完全性 [%] | 67.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | modified microbatch | 5.6 | 291 | PEG 4000, isopropanol, sodium citrate, pH 5.6, modified microbatch, temperature 291K |
