1NMC
COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 15 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1995-07 |
| Detector | PHOTON FACTORY |
| Spacegroup name | P 4 21 2 |
| 格子定数 [Å] | 144.400, 144.400, 227.000 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 7.000 - 2.500 |
| R因子 | 0.22 |
| Rwork | 0.220 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nca |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 26.400 * |
| Data reduction software | PROTEIN |
| Data scaling software | PROTEIN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 2.600 | |
| 分解能 [Å] (高) | 2.500 | 2.500 |
| Rmerge_l_obs | 0.104 | |
| Total number of observations | 156708 * | |
| 独立反射数 | 67425 | |
| 完全性 [%] | 80.0 | 70 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 6.6 | Malby, R.L., (1993) Proteins: Struct.,Funct., Genet., 16, 57. * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | NC10scFv | 5 (mg/ml) | |
| 2 | 1 | drop | N9NA | 10 (mg/ml) | |
| 3 | 1 | drop | potassium phosphate | 1.7 (M) | |
| 4 | 1 | reservoir | phosphate | 1.3 (M) |
