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1NKH

Crystal structure of Lactose synthase complex with UDP and Manganese

Replaces:  1J8X
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X9B
Synchrotron siteNSLS
BeamlineX9B
Temperature [K]100
Detector technologyCCD
Collection date2000-08-08
DetectorADSC QUANTUM 4
Wavelength(s)0.9800
Spacegroup nameP 1 21 1
Unit cell lengths55.469, 99.145, 102.261
Unit cell angles90.00, 104.14, 90.00
Refinement procedure
Resolution24.710 - 2.000
R-factor0.196
Rwork0.196
R-free0.29000

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Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.006

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RMSD bond angle1.500

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Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]25.0002.170
High resolution limit [Å]2.0002.000
Rmerge0.082

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Total number of observations224308

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Number of reflections71688

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<I/σ(I)>151.1
Completeness [%]99.0

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77
Redundancy2.24
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.6

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298PEG 4000, sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropbeta4Gal-T120 (mg/ml)
21dropLA10 (mg/ml)
31reservoir100 (mM)
41reservoirsodium citrate100 (mM)pH5.6
51reservoirPEG400012.5 (%(w/v))
61drop17 (mM)

217705

数据于2024-03-27公开中

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