1NGP

N1G9 (IGG1-LAMBDA) FAB FRAGMENT COMPLEXED WITH (4-HYDROXY-3-NITROPHENYL) ACETATE

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (2.4 Å)

Cell axes110.691110.69198.250
Cell angles90.0090.0090.00
SpacegroupP 41 21 2
Resolution limits15.00 - 2.40
the highest resolution shell value -
R-factor0.196*
R-work0.19600
R-free0.25000
RMSD bond length0.015
RMSD bond angle0.039

Data Collection Statistics

Resolution limits19.00 (15.00*) - 2.40
the highest resolution shell value2.60* - 2.40*
Number of reflections21761
Number of measurements76319*
Rmerge_l_obs0.066
Completeness89.3 (89.0*)
the highest resolution shell value68.*
Redundancy3.51
I/sigma(I)0

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1Vapor diffusion, hanging drop*8.5*10**

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirammonium salfate1.8-1.9 (M)
21reservoirTris0.1 (M)
31dropFab solution10 (mg/ml)
41dropammonium salfate1.8-1.9 (M)
51dropTris0.1 (M)
Annotated Information is extracted from Literature Info*