1NGP

N1G9 (IGG1-LAMBDA) FAB FRAGMENT COMPLEXED WITH (4-HYDROXY-3-NITROPHENYL) ACETATE

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY
Synchrotron site	Photon Factory
Detector technology	IMAGE PLATE
Collection date	1994-02-22
Detector	SATOW
Spacegroup name	P 41 21 2
格子定数 [Å]	110.691, 110.691, 98.250
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	15.000 - 2.400
R因子	0.196 *
Rwork	0.196
R-free	0.25000
結合長の平均二乗偏差(RMSD) [Å]	0.015
結合角の平均二乗偏差(RMSD) [度]	0.039
Data reduction software	OSC
Phasing software	X-PLOR (2.1)
Refinement software	PROLSQ

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	15.000 *
分解能 [Å] (高)	2.400	2.400 *
Rmerge_l_obs	0.066
Total number of observations	76319 *
独立反射数	21761
完全性 [%]	89.0 *	68 *
冗長性	3.51

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	8.5 *	10 *

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	reservoir	ammonium salfate	1.8-1.9 (M)
2	1	reservoir	Tris	0.1 (M)
3	1	drop	Fab solution	10 (mg/ml)
4	1	drop	ammonium salfate	1.8-1.9 (M)
5	1	drop	Tris	0.1 (M)