1NDC

X-RAY STRUCTURE OF NUCLEOSIDE DIPHOSPHATE KINASE COMPLEXED WITH DTDP AND MG2+ AT 2 A RESOLUTION

Experimental procedure

Spacegroup name	H 3 2
Unit cell lengths	79.800, 79.800, 160.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.000 - 2.000
R-factor	0.183
Rwork	0.183
RMSD bond length	0.009
RMSD bond angle	1.600
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	16.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.068 *
Total number of observations	71549 *
Number of reflections	13562 *
Completeness [%]	99.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		(mM)
2	1	drop	Tris-HCl	(mM)
3	1	drop	MdTDP	(mM)
4	1	drop	PEG6000	8 (%)
5	1	reservoir	PEG6000	16 (%)
6	1	drop	protein	5 (mg/ml)