1NAF
Crystal structure of the human GGA1 GAT domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-15 |
| Wavelength(s) | 0.9796, 0.9798, 0.9393 |
| Spacegroup name | P 63 |
| Unit cell lengths | 82.700, 82.700, 69.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.2484 |
| Rwork | 0.248 |
| R-free | 0.28300 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.180 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.066 | 0.399 |
| Number of reflections | 6684 | |
| <I/σ(I)> | 14.2 | 2.6 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4.5 | 289 | sodium acetate, lithium sulphate, NaH2PO4/K2HPO4, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dithiothreitol | 5 (mM) | |
| 2 | 1 | drop | protein | 10-15 (mg/ml) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.5 |
| 4 | 1 | reservoir | sodium phosphate | 0.3 (M) | |
| 5 | 1 | reservoir | potassium phosphate | 0.45 (M) | |
| 6 | 1 | reservoir | 0.2 (M) |
