1NA1
The structure of HRV14 when complexed with Pleconaril
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-14 |
| Detector | FUJI |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 437.160, 437.160, 437.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.300 |
| R-factor | 0.223 |
| Rwork | 0.222 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.540 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | ENVELOPE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.164 * | 0.477 * |
| Total number of observations | 545390 * | |
| Number of reflections | 320952 | |
| Completeness [%] | 77.8 | 68.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, NaCl, CaCl2, PEG8K, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 0.25 (M) | |
| 2 | 1 | reservoir | 0.25 (M) | ||
| 3 | 1 | reservoir | 0.1 (M) | ||
| 4 | 1 | reservoir | PEG8000 | 0.3 (%) | |
| 5 | 1 | drop | protein | 20000 (mg/ml) |
