1N52
Cap Binding Complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2002-01-17 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 111.681, 111.681, 177.588 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 * - 2.120* |
Rwork | 0.223 |
R-free | 0.24270 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h6k |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 * | 2.170 |
High resolution limit [Å] | 2.120 | 2.110 |
Rmerge | 0.058 * | |
Number of reflections | 78602 * | |
<I/σ(I)> | 16 | 2.4 |
Completeness [%] | 99.0 * | |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 4 * | PEG 400, magnesium chloride, Tris, Glycine, Ethylene Glycol , pH 7.25, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 4 * | PEG 400, magnesium chloride, Tris, Glycine, Ethylene Glycol , pH 7.25, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | reservoir | PEG400 | 22 (%(w/v)) | |
3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.25 |
4 | 1 | reservoir | 125 (mM) | ||
5 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
6 | 1 | reservoir | dithiothreitol | 2 (mM) | |
7 | 1 | reservoir | glycine | 10 (mM) | |
8 | 1 | reservoir | ethylene glycol | 3 (%(v/v)) |