1MWC

WILD TYPE MYOGLOBIN WITH CO

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	150
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS
Spacegroup name	I 1 21 1
格子定数 [Å]	122.180, 42.170, 91.670
格子定数 [度]	90.00, 92.61, 90.00

精密化法

残基	24.000 - 1.700
R因子	0.189 *
Rwork	0.189
R-free	0.23700
Structure solution method	OTHER
結合長の平均二乗偏差(RMSD) [Å]	0.020
結合角の平均二乗偏差(RMSD) [度]	0.035
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	CCP4
Refinement software	REFMAC

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	24.000
分解能 [Å] (高)	1.700
Rmerge_l_obs	0.038	0.249 *
独立反射数	48028
<I/σ(I)>	23.4
完全性 [%]	98.0 *
冗長性	6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	7.1		0.1 PHOSPHATE BUFFER PH 7.1 10 MG/ML PROTEIN

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	ammonium sulfate	72-80 (%(v/v)sat)
2	1	drop	phosphate	0.1 (M)
3	1	drop	protein	10 (mg/ml)