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1MUM

Structure of the 2-Methylisocitrate Lyase (PrpB) from Escherichia coli

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2002-02-05
DetectorRIGAKU RAXIS IV
Wavelength(s)1.5418
Spacegroup nameP 32 2 1
Unit cell lengths82.895, 82.895, 166.250
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution30.000

*

- 1.900
R-factor0.218
Rwork0.213
R-free0.23400
Structure solution methodMIR
RMSD bond length0.005
RMSD bond angle22.200

*

Data scaling softwareSCALEPACK
Phasing softwareSOLVE
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.000

*

1.970
High resolution limit [Å]1.9001.900
Rmerge0.0540.370
Total number of observations255945

*

Number of reflections52996
<I/σ(I)>20.53
Completeness [%]98.298.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, sitting drop

*

7

*

18

*

ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoir0.02 (%(w/v))
21dropprotein15 (g/l)
31dropHEPES10 (mM)pH7.0

217705

건을2024-03-27부터공개중

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