1MUM
Structure of the 2-Methylisocitrate Lyase (PrpB) from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-02-05 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 82.895, 82.895, 166.250 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 * - 1.900 |
R-factor | 0.218 |
Rwork | 0.213 |
R-free | 0.23400 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 22.200 * |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.054 | 0.370 |
Total number of observations | 255945 * | |
Number of reflections | 52996 | |
<I/σ(I)> | 20.5 | 3 |
Completeness [%] | 98.2 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 * | 18 * | ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 0.02 (%(w/v)) | ||
2 | 1 | drop | protein | 15 (g/l) | |
3 | 1 | drop | HEPES | 10 (mM) | pH7.0 |