1MNS
ON THE ROLE OF LYSINE 166 IN THE MECHANISM OF MANDELATE RACEMASE FROM PSEUDOMONAS PUTIDA: MECHANISTIC AND CRYSTALLOGRAPHIC EVIDENCE FOR STEREOSPECIFIC ALKYLATION BY (R)-ALPHA-PHENYLGLYCIDATE
Experimental procedure
Spacegroup name | I 4 2 2 |
格子定数 [Å] | 125.000, 125.000, 105.600 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 6.000 * - 2.000 |
R因子 | 0.153 |
Rwork | 0.153 |
結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
結合角の平均二乗偏差(RMSD) [度] | 2.600 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Quality characteristics
Overall | |
分解能 [Å] (高) | 2.000 * |
Rmerge_l_obs | 0.075 * |
Total number of observations | 62094 * |
完全性 [%] | 83.0 * |
冗長性 | 2.6 * |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion, hanging drop * | 8 * | taken from Neidhart, D.J. et al (1991). Biochemistry, 30, 9264-9273. * |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | enzyme | 3 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | 0.01 (%) | ||
5 | 1 | reservoir | ammonium sulfate | 30 (%sat) |