1MMS
Crystal structure of the ribosomal PROTEIN L11-RNA complex
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | BRANDEIS |
| Wavelength(s) | 0.980,1.010 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 63.890, 84.260, 155.510 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 20.000 - 2.570 |
| R因子 | 0.228 * |
| Rwork | 0.219 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.851) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 2.730 |
| 分解能 [Å] (高) | 2.570 | 2.570 |
| 独立反射数 | 49313 | |
| <I/σ(I)> | 20 | 4.3 |
| 完全性 [%] | 95.5 | 86.2 |
| 冗長性 | 4 | 3.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | 25% GLYCEROL, 15% PEG 4000, 50 MM TRIS PH 7.5, 50 MM MGCL2, 20 MM CDCL2, 0.2 M KCL, 1 MM DITHIOTHREITOL, 4 DEGREES C, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | GLYCEROL | ||
| 2 | 1 | 1 | PEG 4000 | ||
| 3 | 1 | 1 | TRIS | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | CDCL2 | ||
| 6 | 1 | 1 | KCL | ||
| 7 | 1 | 1 | DITHIOTHREITOL | ||
| 8 | 1 | 2 | PEG 4000 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 0.15 (mM) | |
| 2 | 1 | reservoir | glycerol | 25 (%) | |
| 3 | 1 | reservoir | PEG4000 | 15 (%) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | 50 (mM) | ||
| 6 | 1 | reservoir | 20 (mM) | ||
| 7 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 8 | 1 | reservoir | Tris | 50 (mM) |
