1MG6

The Crystal Structure of a K49 PLA2 from the Snake Venom of Agkistrodon acutus

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OTHER
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1999-04-17
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
格子定数 [Å]	45.300, 59.550, 46.130
格子定数 [度]	90.00, 117.69, 90.00

精密化法

残基	30.000 - 1.600
R因子	0.221
Rwork	0.197
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1clp
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	30.000	1.630
分解能 [Å] (高)	1.600	1.600
Rmerge_l_obs	0.051	0.226
独立反射数	13205
完全性 [%]	91.8	77.3

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	20%PEG4000, 20%Isopropanol, 0.1M tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K