1MC0
Regulatory Segment of Mouse 3',5'-Cyclic Nucleotide Phosphodiesterase 2A, Containing the GAF A and GAF B Domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-06-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0332 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 134.200, 136.200, 149.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.860 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.26900 * |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 21.600 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.970 | 2.970 |
High resolution limit [Å] | 2.860 | 2.860 |
Rmerge | 0.058 * | 0.408 * |
Number of reflections | 15282 | |
Completeness [%] | 95.9 | 87.2 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 294 | 1,4 butanediol, sodium acetate, dithiothreitol, EDTA, Tris, sodium chloride, 3',5' guanosine monophosphate, leupeptin, pepstatin, phenylmethylsulfonyl flouride, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2-5 (mg/ml) | |
10 | 1 | reservoir | 1,4butanediol | 16-26 (%) | |
11 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.0 |
12 | 1 | reservoir | beta-mercaptoethanol | 140 (mM) | |
2 | 1 | drop | 20 (mM) | ||
3 | 1 | drop | Tris | 10 (mM) | pH7.5 |
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | drop | EDTA | 0.1 (mM) | |
6 | 1 | drop | PMSF | 0.1 (mM) | |
7 | 1 | drop | leupeptin | 0.001 (mg/ml) | |
8 | 1 | drop | pepstain A | 0.001 (mg/ml) | |
9 | 1 | drop | Na 3',5'cGMP | 2 (mM) |