1M6C

V68N MYOGLOBIN WITH CO

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	150
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS
Spacegroup name	I 1 21 1
格子定数 [Å]	122.140, 42.300, 91.550
格子定数 [度]	90.00, 92.49, 90.00

精密化法

残基	24.000 - 1.900
R因子	0.192 *
Rwork	0.192
R-free	0.26100
Structure solution method	OTHER
結合長の平均二乗偏差(RMSD) [Å]	0.019
結合角の平均二乗偏差(RMSD) [度]	0.037
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	CCP4
Refinement software	REFMAC

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	24.000
分解能 [Å] (高)	1.900
Rmerge_l_obs	0.042 *	0.214 *
独立反射数	35786
<I/σ(I)>	22.3
完全性 [%]	94.7 *
冗長性	4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	7.1		0.1 PHOSPHATE BUFFER PH 7.1 10 MG/ML PROTEIN

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	ammonium sulfate	72-80 (%(v/v)sat)
2	1	drop	phosphate	0.1 (M)
3	1	drop	protein	10 (mg/ml)