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1LZX

Rat neuronal NOS heme domain with NG-hydroxy-L-arginine bound

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL7-1
Synchrotron siteSSRL
BeamlineBL7-1
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2001-01-08
DetectorMARRESEARCH
Wavelength(s)1.08
Spacegroup nameP 21 21 21
Unit cell lengths51.930, 110.740, 164.730
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution31.580 - 2.000
Rwork0.226
R-free0.25600
Structure solution methodFOURIER SYNTHESIS
RMSD bond length0.007
RMSD bond angle22.700

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.030
High resolution limit [Å]2.0002.000
Rmerge0.0690.780
Total number of observations208445

*

Number of reflections62410
<I/σ(I)>8.82.1
Completeness [%]96.192.5
Redundancy3.32.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP65

*

PEG4000, MES, ammonium acetate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 280K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein7-9 (mg/ml)
21dropimidazole5 (mM)
31reservoirPEG335018-22 (%(w/v))
41reservoirMES0.1 (M)pH5.6-6.0
51reservoirammonium acetate100-200 (mM)
61reservoirGSH2.0 (mM)
71reservoirSDS35000 (mM)

217705

數據於2024-03-27公開中

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