1LXN
X-RAY STRUCTURE OF MTH1187 NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET TT272
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 175.365, 175.365, 175.365 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.300 |
| Rwork | 0.216 |
| R-free | 0.24500 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.058 * | |
| Total number of observations | 425012 * | |
| Number of reflections | 40380 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 * | 4 * | |
| 1 | Vapor diffusion * | 8.5 * | 4 * | |
| 1 | Vapor diffusion * | 8.5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | |
| 2 | 1 | drop | 500 (mM) | ||
| 3 | 1 | drop | protein | 20 (mg/ml) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 5 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 6 | 1 | reservoir | glycerol | 14 (%) |
