1LXJ
X-RAY STRUCTURE OF YBL001c NORTHEAST STRUCTURAL GENOMICS (NESG) CONSORTIUM TARGET YTYst72
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97895 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 67.380, 67.380, 133.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.800 |
Rwork | 0.211 |
R-free | 0.24300 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 23.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.096 * | |
Total number of observations | 141589 * | |
Number of reflections | 12726 | |
Completeness [%] | 86.8 | 85.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.5 * | 4 * | Ammonium Sulfate, 2-METHYL-2,4-PENTANEDIOL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 10 (mM) | |
2 | 1 | drop | 500 (mM) | ||
3 | 1 | drop | protein | 20 (mg/ml) | |
4 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |
5 | 1 | reservoir | ammonium sulfate | 2 (M) | |
6 | 1 | reservoir | MPD | 12 (%) |