1LTW
RECOMBINANT SPERM WHALE MYOGLOBIN 29W MUTANT (OXY)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 6 |
| 格子定数 [Å] | 91.461, 91.461, 45.841 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 5.000 - 1.700 |
| R因子 | 0.158 |
| Rwork | 0.158 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.018 |
| 結合角の平均二乗偏差(RMSD) [度] | 20.560 * |
| Data reduction software | RIGAKU |
| Data scaling software | RIGAKU |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 30.000 |
| 分解能 [Å] (高) | 1.600 |
| Rmerge_l_obs | 0.087 |
| 独立反射数 | 17658 |
| 完全性 [%] | 71.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | unknown * | 7.4 * | pH 9 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | 1 | ammonium salfate | 2.2-2.6 (M) | |
| 2 | 1 | 1 | Tris-HCl | 20 (mM) | |
| 3 | 1 | 1 | EDTA | 1 (mM) | |
| 4 | 1 | 2 | sodium phosphate | 50 (mM) | pH7.4 |
