1LR4

Room Temperature Crystal Structure of the Apo-form of the catalytic subunit of protein kinase CK2 from Zea mays

「1A6O」から置き換えられました

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-10-02
Detector	MARRESEARCH
Wavelength(s)	1.1
Spacegroup name	C 1 2 1
格子定数 [Å]	142.550, 61.440, 45.410
格子定数 [度]	90.00, 103.06, 90.00

精密化法

残基	26.000 - 2.000
R因子	0.193 *
Rwork	0.193
R-free	0.23700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	BACKBONE OF CYCLIN-DEPENDENT KINASE 2 (PDB entry 1HCL)
結合長の平均二乗偏差(RMSD) [Å]	0.016
結合角の平均二乗偏差(RMSD) [度]	1.520
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	REFMAC (5.0)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	26.000 *	2.110
分解能 [Å] (高)	2.000	2.000
Rmerge_l_obs	0.106 *	0.111 *
独立反射数	24342
完全性 [%]	93.7 *	84.9
冗長性	2.8	1.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	8.1 *	20 *	used microseeding *

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	reservoir	PEG3350	20 (%(w/v))
2	1	reservoir	sodium acetate	200 (mM)
3	1	reservoir	Tris-HCl	100 (mM)	pH8.1