1LR4
Room Temperature Crystal Structure of the Apo-form of the catalytic subunit of protein kinase CK2 from Zea mays
「1A6O」から置き換えられましたExperimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10-02 |
Detector | MARRESEARCH |
Wavelength(s) | 1.1 |
Spacegroup name | C 1 2 1 |
格子定数 [Å] | 142.550, 61.440, 45.410 |
格子定数 [度] | 90.00, 103.06, 90.00 |
精密化法
残基 | 26.000 - 2.000 |
R因子 | 0.193 * |
Rwork | 0.193 |
R-free | 0.23700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | BACKBONE OF CYCLIN-DEPENDENT KINASE 2 (PDB entry 1HCL) |
結合長の平均二乗偏差(RMSD) [Å] | 0.016 |
結合角の平均二乗偏差(RMSD) [度] | 1.520 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | X-PLOR |
Refinement software | REFMAC (5.0) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 26.000 * | 2.110 |
分解能 [Å] (高) | 2.000 | 2.000 |
Rmerge_l_obs | 0.106 * | 0.111 * |
独立反射数 | 24342 | |
完全性 [%] | 93.7 * | 84.9 |
冗長性 | 2.8 | 1.7 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, SITTING DROP | 8.1 * | 20 * | used microseeding * |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | reservoir | PEG3350 | 20 (%(w/v)) | |
2 | 1 | reservoir | sodium acetate | 200 (mM) | |
3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.1 |