1LPL

Structural Genomics of Caenorhabditis elegans: CAP-Gly domain of F53F4.3

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-10
Detector	MARRESEARCH
Wavelength(s)	1.74
Spacegroup name	P 61 2 2
格子定数 [Å]	64.156, 64.156, 101.946
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	50.000 * - 1.770
R因子	0.225 *
Rwork	0.220
R-free	0.29400 *
Structure solution method	SAS
結合長の平均二乗偏差(RMSD) [Å]	0.021 *
結合角の平均二乗偏差(RMSD) [度]	1.700 *
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	ISAS (V. 2001)
Refinement software	X-PLOR (3.851)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	1.880
分解能 [Å] (高)	1.770	1.770
Rmerge_l_obs	0.043 *	0.369 *
独立反射数	12651 *
完全性 [%]	99.2 *	95 *
冗長性	9.8 *

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, sitting drop *	7.4 *	298	Reservoir solution contained 1.8 M ammonium sulphate and 0.1 M MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	reservoir	ammonium sulfate	1.8 (M)
2	1	reservoir	MES	0.1 (M)	pH6.5
3	1	drop	protein	10 (mg/ml)
4	1	drop	HEPES	20 (mM)	pH7.4