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1LOA

THREE-DIMENSIONAL STRUCTURES OF COMPLEXES OF LATHYRUS OCHRUS ISOLECTIN I WITH GLUCOSE AND MANNOSE: FINE SPECIFICITY OF THE MONOSACCHARIDE-BINDING SITE

Experimental procedure
Spacegroup nameP 1 21 1
Unit cell lengths56.300, 139.800, 62.700
Unit cell angles90.00, 91.00, 90.00
Refinement procedure
Resolution8.000 - 2.200
R-factor0.179
Rwork0.179
RMSD bond length0.003

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RMSD bond angle3.000
Phasing softwareX-PLOR
Refinement softwareX-PLOR
Data quality characteristics
 Overall
High resolution limit [Å]2.200

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Rmerge0.082

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Total number of observations77034

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Number of reflections33640

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Completeness [%]67.0

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Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, sitting drop

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7.5

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20

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reservoirs contain the same ingredients as drops, but twice as consentrated. taken from Bourne, Y. et al. (1988). J. Mol. Biol., 202, 685-687.

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Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein10 (mg/ml)
21dropMPD40 (%(v/v))
31drop0.05 (M)
41dropHEPES0.02 (M)

217705

건을2024-03-27부터공개중

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