1LJO

CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM2) FROM ARCHAEOGLOBUS FULGIDUS AT 1.95A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	1999-04-29
Detector	MARRESEARCH
Wavelength(s)	1
Spacegroup name	P 6
Unit cell lengths	58.420, 58.420, 32.084
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.100 - 1.950
R-factor	0.195
Rwork	0.194
R-free	0.21100
Structure solution method	SIRAS
Starting model (for MR)	Built by ARP/WARP (68 residues)
RMSD bond length	0.005
RMSD bond angle	1.320 *
Data scaling software	XDS
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.051	0.173
Total number of observations	11984 *
Number of reflections	4522
<I/σ(I)>	10.5	4.2
Completeness [%]	97.6	90.9
Redundancy	2.7	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.3 *	20 *	ammonium sulphate, lithium sulphate, sodium sulphate, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.3 *	20 *	ammonium sulphate, lithium sulphate, sodium sulphate, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.3 *	20 *	ammonium sulphate, lithium sulphate, sodium sulphate, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	reservoir	PEG6000	12 (%(w/v))
3	1	reservoir	sodium citrate	100 (mM)	pH4.3