1LDC

X-RAY STRUCTURE OF TWO COMPLEXES OF THE Y143F FLAVOCYTOCHROME B2 MUTANT CRYSTALLIZED IN THE PRESENCE OF LACTATE OR PHENYL-LACTATE

Experimental procedure

Collection date	1993-01-08
Spacegroup name	P 32 2 1
Unit cell lengths	164.500, 164.500, 114.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.900
R-factor	0.195
Rwork	0.195
RMSD bond length	0.010
RMSD bond angle	3.700
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.900 *
Rmerge	0.150
Total number of observations	156473 *
Number of reflections	30412
Completeness [%]	80.0
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	10-12 (%(w/w))
2	1	reservoir	MES	50 (mM)