1LBE

APLYSIA ADP RIBOSYL CYCLASE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 21
格子定数 [Å]	70.200, 72.300, 125.200
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	8.000 - 2.400
R因子	0.214
Rwork	0.214
R-free	0.29900
結合長の平均二乗偏差(RMSD) [Å]	0.017
結合角の平均二乗偏差(RMSD) [度]	2.400
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Quality characteristics

	Overall
分解能 [Å] (高)	2.400 *
Rmerge_l_obs	0.103
Total number of observations	84127 *
独立反射数	25029 *
完全性 [%]	98.3
冗長性	3.36

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion *	7 *	18 *	drop solution was mixed with an equal volume of reservoir solution *

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	8.0 (OD280)
2	1	drop		40 (mM)
3	1	drop	HEPES	10 (mM)
4	1	reservoir	PEG4000	30 (%(w/v))
5	1	reservoir	imidazole-malate	0.2 (M)