1KPM
First Structural Evidence of a Specific Inhibition of Phospholipase A2 by Vitamin E and its Implications in Inflammation: Crystal Structure of the Complex Formed between Phospholipase A2 and Vitamin E at 1.8 A Resolution.
Replaces: 1FE7Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 180 |
Detector technology | IMAGE PLATE |
Collection date | 1999-06-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.790, 88.510, 78.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cl5 |
RMSD bond length | 0.014 * |
RMSD bond angle | 2.000 * |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.038 * | 0.084 * |
Number of reflections | 23652 | |
<I/σ(I)> | 9.93 | 2.1 |
Completeness [%] | 96.0 * | 99 |
Redundancy | 6.6 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.5 | 298 | 20mM Sodium cacodylate, 1.4M Ammonium sulfate, 4mM Calcium chloride, 3% dioxane, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | sodium cacodylate | 20 (mM) | pH6.5 |
3 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
4 | 1 | reservoir | dioxane | 3 (%(v/v)) |