1K9X
Structure of Pyrococcus furiosus carboxypeptidase Apo-Yb
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000, 1.3860 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 66.342, 86.780, 107.895 |
| 格子定数 [度] | 88.64, 78.54, 69.44 |
精密化法
| 残基 | 48.780 - 2.300 |
| R因子 | 0.212 |
| Rwork | 0.212 |
| R-free | 0.26700 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.237 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.340 |
| 分解能 [Å] (高) | 2.300 | 2.300 |
| Rmerge_l_obs | 0.036 | 0.087 |
| Total number of observations | 353793 * | |
| 独立反射数 | 98094 | |
| <I/σ(I)> | 26.2 | |
| 完全性 [%] | 93.8 | 80.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG 4000, TRIS, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 19 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH8.0 |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | reservoir | PEG4000 | 25-30 (%) | |
| 5 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
| 6 | 1 | reservoir | 20-40 (mM) |
