1K5M

Crystal Structure of a Human Rhinovirus Type 14:Human Immunodeficiency Virus Type 1 V3 Loop Chimeric Virus MN-III-2

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	108
Detector technology	CCD
Collection date	1998-02-01
Detector	NONIUS CAD4
Wavelength(s)	1.10
Spacegroup name	I 2 2 2
格子定数 [Å]	318.900, 349.300, 368.400
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	50.000 - 2.700
Rwork	0.216
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Wild-type HRV14 structure coordinates (PDB entry 4RHV)
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	26.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.800
分解能 [Å] (高)	2.700	2.700
Rmerge_l_obs	0.114	0.212
Total number of observations	7100958 *
独立反射数	513239
<I/σ(I)>	10	4
完全性 [%]	92.4 *	81.6 *
冗長性	13.8	5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.5 M ammonium formate and 0.15 M sodium HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.5 M ammonium formate and 0.15 M sodium HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	reservoir	ammonium formate	1.5 (M)
2	1	reservoir	sodium HEPES	0.15 (M)	pH7.5