1JZW
Arsenate Reductase + Sodium Arsenate From E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2000-09-07 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 86.790, 86.790, 116.320 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.760 |
R-factor | 0.163 * |
Rwork | 0.164 |
R-free | 0.19200 * |
Structure solution method | AB INITIO |
RMSD bond length | 0.012 |
RMSD bond angle | 0.031 * |
Data scaling software | X-GEN |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.107 | 0.330 |
Total number of observations | 722598 * | |
Number of reflections | 22762 | |
<I/σ(I)> | 21.232 | 8.51 |
Completeness [%] | 84.8 | 40 |
Redundancy | 31.74 | 8.02 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 5 * | 50% saturated Cesium Sulfate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 1.2 (M) | ||
2 | 1 | drop | ammonium acetate | 100 (mM) | pH4.8 |
3 | 1 | drop | protein | 30 (mg/ml) |