1JUO
Crystal Structure of Calcium-free Human Sorcin: A Member of the Penta-EF-Hand Protein Family
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| 格子定数 [Å] | 118.250, 118.250, 85.490 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 37.560 - 2.200 |
| R因子 | 0.212 * |
| Rwork | 0.218 |
| R-free | 0.25600 * |
| Structure solution method | MIR |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 * |
| 結合角の平均二乗偏差(RMSD) [度] | 1.199 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNX (2000) |
| Refinement software | CNX (2000) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.260 |
| 分解能 [Å] (高) | 2.200 | 2.200 |
| Rmerge_l_obs | 0.064 | 0.491 |
| Total number of observations | 167295 * | |
| 独立反射数 | 28472 | |
| <I/σ(I)> | 2.1 | |
| 完全性 [%] | 90.7 | 75.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | sodium chloride, HEPES, EGTA, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | sorcin | 0.004 (mM) | |
| 2 | 1 | drop | fluo-4 pentapossium salt | 0.010 (mM) | |
| 3 | 1 | drop | urea | 6.4 (mM) | or 7.8M |
| 4 | 1 | reservoir | 0.5 (M) | ||
| 5 | 1 | reservoir | HEPES | 0.1 | pH7.0 |
| 6 | 1 | reservoir | EGTA | 2.5 (mM) |
