1JS0

Crystal Structure of 3D Domain-swapped RNase A Minor Trimer

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-10
Detector	ADSC QUANTUM 4
Wavelength(s)	1.072
Spacegroup name	P 21 21 2
格子定数 [Å]	114.590, 122.170, 26.840
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	10.000 - 2.200
R因子	0.184 *
Rwork	0.184
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rtb
結合長の平均二乗偏差(RMSD) [Å]	0.014
結合角の平均二乗偏差(RMSD) [度]	1.906 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	40.000 *	2.180
分解能 [Å] (高)	2.100	2.100
Rmerge_l_obs	0.096 *
Total number of observations	327545 *
独立反射数	22989
<I/σ(I)>	18.6	4.4
完全性 [%]	99.7	99.8
冗長性	14	5.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.7 *	4 *	PEG 10,000, amonium sulfate, pH 3.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	10 (mg/ml)
2	1	drop	phosphate	0.18 (M)	pH6.7