1JL2

Crystal structure of TCEO RNase H-a chimera combining the folding core from T. thermophilus RNase H and the remaining region of E. coli RNase H

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-28
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
格子定数 [Å]	56.195, 87.033, 60.687
格子定数 [度]	90.00, 91.87, 90.00

精密化法

残基	20.000 - 1.760
R因子	0.249 *
Rwork	0.249
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f21
結合長の平均二乗偏差(RMSD) [Å]	0.005 *
結合角の平均二乗偏差(RMSD) [度]	1.250 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.000	1.830
分解能 [Å] (高)	1.760	1.760
Rmerge_l_obs	0.061	0.277
Total number of observations	62607 *
独立反射数	5255 *	189 *
完全性 [%]	97.7	95.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	8	298	50 mM Tris, pH 8.0, 18% PEG600, VAPOR DIFFUSION, HANGING DROP, temperature 298K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	Tris	50 (mM)	pH8.0
3	1	reservoir	PEG600	18 (%)