1JF2
Crystal Structure of W92F obelin mutant from Obelia longissima at 1.72 Angstrom resolution
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-04-08 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
格子定数 [Å] | 53.449, 53.449, 144.485 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 26.280 - 1.720 |
R因子 | 0.232 |
Rwork | 0.232 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1el4 |
結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
結合角の平均二乗偏差(RMSD) [度] | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 30.000 | 1.760 |
分解能 [Å] (高) | 1.700 | 1.700 |
Rmerge_l_obs | 0.037 * | 0.080 * |
独立反射数 | 21633 | |
<I/σ(I)> | 18.08 | |
完全性 [%] | 99.9 * | 87.7 |
冗長性 | 5.8 * | 10.2 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | Deng, L., (2001) FEBS Lett., 506, 281. * |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein | 8-10 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 18 (%) | |
3 | 1 | reservoir | potassium phosphate | 50 (mM) | |
4 | 1 | drop | hexamine cobalt chloride |