1J04
Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 89.491, 89.491, 142.823 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.400 - 2.600 |
Rwork | 0.223 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h0c |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.680 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.092 | 0.487 |
Number of reflections | 18539 | |
<I/σ(I)> | 11.6 | 2.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.6 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 283 | PEG 4000, Na HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |