1IUB
Fucose-specific lectin from Aleuria aurantia (Hg-derivative form)
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-07-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00901, 1.00394, 0.918326 |
| Spacegroup name | P 65 2 2 |
| 格子定数 [Å] | 83.909, 83.909, 254.131 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 40.000 * - 2.310 |
| Rwork | 0.156 |
| R-free | 0.18300 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 25.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 200.000 * | 2.360 |
| 分解能 [Å] (高) | 2.310 | 2.310 |
| Rmerge_l_obs | 0.067 | 0.290 |
| 独立反射数 | 43457 | |
| <I/σ(I)> | 27.2 | |
| 完全性 [%] | 95.4 | 93 |
| 冗長性 | 11.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 297 * | Fujihashi, M., (2003) Acta Crystallogr.,Sect.D, 59, 378. * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | sodium phosphate | 8 (mM) | |
| 3 | 1 | reservoir | potassium phosphate | 1.5 (mM) | |
| 4 | 1 | reservoir | 137 (mM) | ||
| 5 | 1 | reservoir | 2.7 (mM) | pH7.2 | |
| 6 | 1 | reservoir | 1 (mM) |
