1IMH
TonEBP/DNA COMPLEX
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 59.568, 95.368, 158.431 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 30.000 * - 2.860 |
| R因子 | 0.244 |
| Rwork | 0.244 |
| R-free | 0.29000 |
| Structure solution method | MIRAS |
| Starting model (for MR) | 1a02 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.930 |
| 分解能 [Å] (高) | 2.850 | 2.850 |
| Rmerge_l_obs | 0.064 | 0.676 |
| 独立反射数 | 20432 | |
| <I/σ(I)> | 18.2 | 2.77 |
| 完全性 [%] | 93.9 | 99.9 |
| 冗長性 | 12 | 5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PEG 3000, SODIUM CHLORIDE, MAGNESIUM CHLORIDE, BIS-TRIS PROPANE, SODIUM AZIDE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PEG 3000, SODIUM CHLORIDE, MAGNESIUM CHLORIDE, BIS-TRIS PROPANE, SODIUM AZIDE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PEG 3000, SODIUM CHLORIDE, MAGNESIUM CHLORIDE, BIS-TRIS PROPANE, SODIUM AZIDE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | PEG 3000 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | BIS-TRIS PROPANE | ||
| 5 | 1 | 1 | SODIUM AZIDE |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | bis-Tris-BTP-HCl | 50 (mM) | pH6.6 |
| 2 | 1 | reservoir | 100 (mM) | ||
| 3 | 1 | reservoir | PEG3000 | 3-6 (%(w/v)) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | drop | protein | 0.2-0.4 (mM) | |
| 6 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 7 | 1 | drop | dithiothreitol | 1 (mM) | |
| 8 | 1 | drop | 100 (mM) |
