1IDE
ISOCITRATE DEHYDROGENASE Y160F MUTANT STEADY-STATE INTERMEDIATE COMPLEX (LAUE DETERMINATION)
Experimental procedure
| 実験手法 | LAUE |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-10-15 |
| Detector | FUJI |
| Wavelength(s) | 0.7 - 2.1 |
| Spacegroup name | P 43 21 2 |
| 格子定数 [Å] | 105.100, 105.100, 150.300 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 10.000 * - 2.500 |
| R因子 | 0.192 |
| Rwork | 0.192 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.019 |
| 結合角の平均二乗偏差(RMSD) [度] | 26.200 * |
| Data reduction software | OXFORD (LAUE PACKAGE (J.CAMPBELL)) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 100.000 * |
| 分解能 [Å] (高) | 2.500 * |
| Rmerge_l_obs | 0.084 |
| 独立反射数 | 17043 |
| 完全性 [%] | 61.0 |
| 冗長性 | 17.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | unknown * | 5.4 * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | dephosphorylated IDH | 28 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 34 (%sat) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | 35 (mM) | ||
| 5 | 1 | reservoir | citric acid | 9 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 0.2 (mM) |
